WebSep 27, 2024 · The target-specified guide RNA (gRNA) sequences were cloned into a binary vector harbouring the cas9 gene under control of a ubiquitin promoter from parsley (Figure S1; Kawalleck et al ., 1993 ). The binary vectors targeting CsGTR1 and CsGTR2 harboured four gRNAs, while the vector for CsMYB28 and CsMYB29 contained three gRNAs. WebNov 22, 2024 · The pHEE401E_UBQ_Bar vector is a version in which the egg-specific promoter for Cas9 expression in the pHEE401E vector was replaced with the UBQ10 …
pHEE401E - Lifeasible
WebNov 22, 2024 · The vectors used were pHEE401E_UBQ_Bar and pBAtC_tRNA, which employ a one-promoter/one-sgRNA and a polycistronic-tRNA-gRNA strategy, respectively. Golden … WebpHEE401E (Plasmid #71287) Print Enlarge View all sequences Purpose Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for … Plasmid pHEE401 from Dr. Qi-Jun Chen's lab contains the inserts sgRNA scaffold … Plasmid pCBC-DT1T2 from Dr. Qi-Jun Chen's lab contains the insert T1, U626t … philippine international balloon festival
The Tomato Transcription Factor SlNAC063 Is Required for …
WebpHEE401E vector Sequence Copy Sequence Download GeneBank File(.gb) LOCUS Exported 17112 bp ds-DNA circular SYN 13-MAY-2024 DEFINITION Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target ACCESSION . VERSION . WebMar 15, 2024 · Then the fragment was ligated into the binary vector pHEE401E by the restriction-ligation system ( Wang et al., 2015 ). Then, the recombinant plasmid pHEE401E-2sgRNA-SlNAC063 was transformed into wild-type tomato AC using the stable A. tumefaciens -mediated transformation method ( McCormick et al., 1986; Gupta and Van … WebpHEE401E (~100 ng/μl) 2 μl 10× T4 DNA Ligase Buffer (NEB) 1.5 μl 10× BSA 1.5 μl BsaI (NE B) 1 μl T4 DNA Ligase (HC, NEB) 1 μl ddH 2 O 6 μl Total volume 15 μl 5. Transform E.coli … philippine interisland shipping association